Professor Guanghui Wang and Haigang Ren's group discover that glucosylceramide accumulation in microglia triggers STING-dependent neuroinflammation and neurodegeneration

发布者：邓泮发布时间：2024-04-07浏览次数：241

The glucocerebrosidase(GCase) protein, encoded by theGBAgene and localized in lysosomes, is responsible for the degradation of intracellular β-glucosylceramides (GCs). Mutations in theGBAgene are known to be the causative genetic factors for Gaucher disease (GD), a lysosomal storage disorder, and are also considered as major risk factors for Parkinson's disease (PD) and Dementia with Lewy bodies (DLB). Clinical studies have shown that 25% of GD patients withGBAmutations are likely to develop PD, and the risk of cognitive impairment in PD patients withGBAmutations is significantly elevated. Previous research has indicated the involvement of neuroinflammation in the pathology of GD and PD, but the role of GCase in regulating neuroinflammation and its molecular mechanisms remain unclear.

Recently, Professors Guanghui Wang and Haigang Ren from ourcollege, in collaboration with Professors Junhai Han and Ming Fang from Southeast University, published a research paper as a cover article titled Glucosylceramide accumulation in microglia triggers STING-dependent neuroinflammation and neurodegeneration in mice inScience Signaling.


This study revealed that the expression of GCase in microglia in the brain is significantlygreaterthan that in neurons. Inhibiting GCase activity in microglia combined with GC treatment leads to lipid accumulation and activation of STING-dependent inflammation. Mechanistically, GC accumulation in microglia causes mitochondrial dysfunction, resulting in leakage of mitochondrial DNA (mtDNA) into the cytoplasm, triggering STING signaling activation and type I interferon-related inflammatory responses. Additionally, GC accumulation in microglia leads to lysosomal damage, impairing the clearance of damaged mitochondria and hinderingtheirdegradation through the lysosomal pathway. Treatment with rapamycin repairs lysosomal damage andmitophagy dysfunction caused by GC accumulation by promoting lysosome biogenesis, thereby ameliorating mitochondrial impairment, cytoplasmic leakage of mtDNA, and STING-dependent inflammatory responses in microglia. Blocking STING activation significantlyinhibitedmicroglia-mediated neuroinflammation andprotectedneurons from neurodegeneration, thereby improving motor behavioral impairments in the mouse model. These findings provide new insights into the pathogenesis of PD and DLB and offer potential drug targets fortheclinical treatment ofthese diseases.



Rui Wang, a doctoral student jointly trained by our college and Southeast University, and Dr. Hongyang Sun, a postdoctoral researcher in our college, are the co-first authors of this study. Professors Guanghui Wang and Haigang Ren from ourcollegeandProfessorsJunhai Han and Ming Fang from Southeast University are cocorresponding authors. This study wassupportedby the National Natural Science Foundation of China (32261133525, 32271039, 32230039, 32070970, and 32170987) and the Priority Academic Program Development of Jiangsu HigherEducation Institutions from Jiangsu Education Department.

Reference:

Rui Wang, Hongyang Sun, Yifan Cao, Zhixiong Zhang, Yajing Chen, Xiying Wang, Lele Liu, Jin Wu, Hao Xu, Dan Wu, Chenchen Mu, Zongbing Hao, Song Qin, Haigang Ren, Junhai Han, Ming Fang, Guanghui Wang.Glucosylceramide accumulation in microglia triggers STING-dependent neuroinflammation and neurodegeneration in mice.Science Signaling.2024, 17(829): eadk8249.

Full-text link:https://www.science.org/stoken/author-tokens/ST-1774/full

Corresponding authors:

GuanghuiWangis a distinguished professorat theCollege of Pharmaceutical Sciences, Soochow University. Wang’slaboratory has focused mainly on themolecular basis of neurodegenerative diseases and neurodegeneration in association with neuroinflammation. In recent years, he has published more than 90 academic papers as correspondingauthorinSci Adv,Nat Commun,EMBO J,Mol Psychiatry,Autophagy,Aging Cell,Cell Death Differ,Brain,Sci SignalandCell Rep.To date, these papers have been cited more than 11,500 times.

Haigang Ren is a professorat theCollege of Pharmaceutical Sciences, Soochow University. Dr. Renfocusesonthemolecular mechanisms of Parkinson’s diseaseandneurodegeneration in association with mitochondrial dysfunction and neuroinflammation. He has published more than 30 academic papers asthecorresponding author or first author inSci Adv,Mol Neurodegener,Sci Signal,Sci China Life Sci,Cancer Lett,J Biol Chem,Acta Pharmacol Sin,Cell Death DisandNeurosci Bull.