Topic:Unravelling cell signaling and nuclear events using the 4C quantitative PTM proteomics and Biomolecular Modulomics
Speaker: Professor Ning Li (The Hong Kong University of Science and Technology, HK, China)
Time: 9:00-10:00, Sep 29, 2023
Location: Conference Room 2108, Yunxuan Building
Abstract
Terrestrial plants have evolved sophisticated sensing systems and flexible adaptive abilities to capture sunlight and to develop resistance to both biotic and abiotic stresses in order to survive in versatile environments. To unravel the cell signal transduction pathways mediating blue light, touch, rain, wind signaling in plants, we have developed the stable isotope labeling-based 4C quantitative PTM proteomics (phosphorpoteomics and biotinylproteomics) and applied them onto a model eukaryotic organism Arabidopsis plant and discovered BLEPH1, TREPH1 and WIRK1 signaling components that play a role either in mechano-response or/and tropic responses of Arabidopsis.
To address the nuclear events occurred in response to stress hormone signals, we also developed a 4C quantitative biomolecular modulomics (or called CHAMPION approach) tocapture both nuclear protein complexes and nuclear bodies, to construct the topological architecture of protein interaction modules and signals-triggered module variants in nucleome and to map the protein compositions of biomolecular condensates present in nucleus. This CHAMPION approach allows us for the first time to test the idea of modular cell biology concepts.
(Supporting grants: 31370315, 31570187, 31870231, 32070205 from the National Science Foundation of China and16102422, 16103621, 16101114, 16103817, 16103615,16100318, 16101819, 16101920 from the RGC of Hong Kong).
References:
1. Shuaijian Dai, Shichang Liu, Chen Zhou, Fengchao Yu, Guang Zhu, Wenhao Zhang, Haiteng Deng, Al Burlingame, Weichuan Yu*, Tingliang Wang*, and Ning Li* (2023) Capturing the hierarchically associated modules of protein interaction in the organized nucleome.Mol Plant. 16, 930-961.
2. Zhu Yang, Guangyu Guo, Nan Yang, Sunny Sing Pun, Timothy Ka Leung Ho, Ling Ji, Inch Hu, Jianhua Zhang, Alma L. Burlingame, Ning Li*（2020）The change of gravity vector induces short-term phosphoproteomic alterations in Arabidopsis.Journal of Proteomics, 218, 103720.
3. Wang, K, Yang, Z, Qing, DJ, Ren, F, Liu, SC, Zheng, QS, Liu, J, Zhang, WP, Dai, C, Wu, M, Chehab, W, Braam, J and Li, Ning* (2018) Quantitative and functional post-translational modification proteomics reveals that TREPH1 plays a role in plant touch-delayed bolting.Proc. Natl. Acad. Sci.115, 10830-10835.
4. LiuS,YuF,Yang Z,Wang T,Xiong H,Chang C,Yu WC*,Li, Ning*. (2018) Establishment of Dimethyl Labeling-based Quantitative Acetylproteomics in Arabidopsis.Mol Cell Proteomics.17，1010-1027.
5. Liu, SC, Yu, FC, Hu, Q, Wang, TL, Yu, LJ, Du, SW, Yu, WC* and Li, Ning*. (2108) Development ofin PlantaChemical Cross-Linking-Based Quantitative Interactomics in Arabidopsis.J. Proteome Research. 17, 3195-3213.
